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SBI EXO-FBS-50A-1-不含外泌體的胎牛血清大量現貨

SBI EXO-FBS-50A-1-不含外泌體的胎牛血清大量現貨供應,2600元一瓶;

System Biosciences
System Biosciences,簡稱SBI,美國加州灣區新成立的生技公司,致力于獨特、創欣生物技術之開發,以研發利于基因及蛋白質功能鑒定、研究之嶄新方法和工具為宗旨。 現階段研發重心為RNA干擾(RNAi)研究之相關工具。 System Biosciences (SBI) 致力于開發獨特、革新的技術,為客戶研究蛋白組學和基因組學功能提供研究工具。SBI 是專業的慢病毒產品公司,提供基于慢病毒的所有相關產品、質粒、試劑盒及相關配套試劑和慢病毒延伸產品如IPS細胞多功能性誘導試劑盒和RNAi篩選文庫。



概觀

避免無意中研究牛外泌體

胎牛血清或FBS是許多類型細胞培養基的重要組成部分。但對于有興趣從培養細胞中分離外泌體的研究人員來說,標準FBS可能會引入不需要的并發癥 - 牛外泌體,這可能會導致嚴重的背景問題或干擾功能研究。這就是為什么SBI開發了Exo-FBS,我們的磚利外泌體耗盡的FBS。

  • 去除外泌體大小的囊泡
  • 非常低水平的CD63陽性牛外泌體
  • 無法檢測到的牛miRNA水平
  • 可比增長率為標準FBS
  • 可與標準FBS互換(在DMEM或RPMI中添加10%)

支持數據

高品質和卓越的性能

Exo-FBS支持強大的細胞生長(圖1),大大降低了牛外泌體(圖2)和牛miRNA(圖3)的水平。補充有Exo-FBS的培養基中的細胞生長類似于補充有標準FBS的培養基中的細胞生長(圖1)。

Exo-FBS支持比競爭對手更強大的細胞生長健康

Exo-FBS支持比競爭對手更強大的細胞增長

圖1. Exo-FBS支持比G公司外泌體耗盡的FBS更強大的細胞生長。HepG2細胞在含有標準FBS(對照),Exo-FBS或Company G的外來體耗盡的FBS的培養基中生長。在一次和兩次傳代后顯示數據,并說明Exo-FBS提供比公司G產品更多數量的活細胞(左圖,使用CCK-8測定評估的活力)和更健康的細胞形態(右圖)。

 

抗-CD63 Elisa在Exo-FBS中顯示低水平的外泌體

 

圖2.牛α-CD63 ELISA顯示Exo-FBS中低水平的外泌體 CD63是外來體特異性標記物。標準FBS和Exo-FBS的α-CD63 ELISA在Exo-FBS中顯示非常低水平的CD63,支持NTA數據,其顯示Exo-FBS中外來體大小的顆粒數量低(圖1)。使用等體積(50μl)的標準FBS或Exo-FBS耗盡的培養基補充物,并將繪制的結果歸一化至標準FBS的信號水平。

 

qPCR顯示在mio-FBS中檢測不到牛miRNA

 

圖3.qPCR測定顯示Exo-FBS中不可檢測水平的牛外泌體miRNA。雖然標準FBS含有可擴增的miRNA(測試的72個單獨miRNA中的12個,左圖),但Exo-FBS顯示無可擴增的miRNA(右圖)。用Trizol提取方法處理標準FBS和Exo-FBS培養基補充物(4ml)以回收外來體RNA。將RNA轉化為cDNA,并使用SBI的QuantiMir系統通過qPCR測量72個單獨的牛microRNA。

文獻:
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